D-limonene in diabetic rats

Introduction: Diabetes mellitus (DM) is a multi-factorial condition associated with oxidative stress. Limonene, as plant-derived antioxidant, can be used for treating DM. Objectives: An investigation on antioxidant effects in diabetic rats exposed to D-limonene. Materials and Methods: Sixty male Wistar were categorized into six groups follows: control (healthy rats), (untreated sham glibenclamide, limonene, finally limonene. Alloxan (100 mg/dL) was infused intraperitoneally induce type 1 diabetes rats. Rats certain given limonene glibenclamide (10 orally 8 weeks. Subsequently, animals killed, their kidneys removed. Serum levels of biochemical factors (including serum creatinine, urea, glucose) determined, such nitric oxide (NO), malondialdehyde (MDA), glutathione (GSH), myeloperoxidase (MPO) measured kidney tissue homogenate. The gene expression enzymatic activity peroxidase (GPx), catalase (CAT), superoxide dismutase (SOD) the by real-time polymerase chain reaction (real-time PCR) spectrophotometry, respectively. Results: Limonene treatment significantly decreased glucose, urea. Additionally, MDA, MPO, NO while GSH increased after Real-time RT-PCR showed significant elevation (P<0.05) mRNA GPx, CAT, SOD limonene-treated compared group. Conclusion: Our results demonstrated that an herbal had better markers rat models diabetes.